The National Federation of Independent Business, which joined Florida and 25 other states in fighting the Affordable Care Act all the way to the U.S. Supreme Court, has begun airing TV spots in the state opposing Medicaid expansion.

The Florida House and Senate have already rejected Gov. Rick Scott’s call for expansion. The Senate is now working on a plan that would rely on the $51 billion promised by the federal government over the next decade to give low-income Floridians vouchers for private insurance in a newly crafted Healthy Florida program.

NFIB challenges whether Florida can rely on the promise that Washington will be there with the money if Medicaid expands. The organization also is trying to get Floridians to sign petitions opposing expansion.

“The expansion of Medicaid will cost Florida taxpayers at least $3.5 billion over 10 years,” said NFIB-Florida executive director Bill Herrle. “These expenses are a cause for concern when it comes to job creation and the viability of our economy.”

The NFIB and the resisting states lost last year when the Supreme Court upheld the federal health care overhaul. But justices left it to states to decide whether they want to opt into the Medicaid expansion.

Scott’s evolution to supporting expansion has cost him support among many conservatives. But lawmakers haven’t ruled out using the federal cash for a state-crafted program.

The National Federation of Independent Business, which early on joined Florida and 25 other states in challenging the Affordable Care Act, condemned the Supreme Court’s decision Thursday that maintains central parts of the sweeping law.

“Florida’s small-business owners have been fighting this battle alongside the Florida Attorney General’s office from day one, and after months of uncertainty and frustration, the Court’s decision is grave disappointment to them,” said Bill Herrle, NFIB-Florida’s executive director.

 “The increasing costs of health-care are the number one concern for the small-business community, and this flawed law does nothing to address cost,” he added.

States are likely to find some silver lining in the ruling, which stops short of imposing strict penalties on those that don’t comply with a major expansion of Medicaid coverage. But for businesses opposed to the measure, there is little to cheer.

 “This day will go down in history as the day when Americans lost a part of their freedom – the freedom to choose what to buy with their money,” said Karen Harned, executive director of NFIB’s Small Business Legal Center.